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nikon a1 r confocal microscope  (Nikon)


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    Nikon nikon a1 r confocal microscope
    Nikon A1 R Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a1+r+confocal+microscope/Objectives/pmc12895044-98-6-6
    Average 99 stars, based on 59633 article reviews
    nikon a1 r confocal microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Software:

    Article Title: Induction of intercrypt goblet cells upon bacterial infection
    Article Snippet: .. Slides were then scanned using Axioscan Z1 Zeiss slide scanner (Carl Zeiss Microscopy GmbH) and images were analyzed with the Zen 2.6 software, or viewed on a Nikon A1 R confocal microscope (Nikon Instruments Inc), and images were taken using NIS elements AR software, and further processed using FiJI (Image J) . .. Mice distal colonic tissues were solubilized in an extraction buffer containing 4 M guanidine chloride, 5 mM ethylenediaminetetraacetic acid (EDTA), 10 mM benzamidine, 5 mM oN-ethylmaleimide, 0.1 mg/mL trypsin inhibitor and 1 mM phenylmethanesulfonyl fluoride) for 24h, mucins were purified by density gradient ultracentrifugation at 417,600 g at 15 °C for 72 h (Beckman Coulter LE80 K ultracentrifuge; 70.1 Ti rotor), and identified by Periodic Acid Schiff (PAS) staining after transfer of the different fractions onto a nitrocellulose membrane by slot-blotting.

    Article Title: Induction of intercrypt goblet cells upon bacterial infection: a promising therapeutic target to restore the mucosal barrier
    Article Snippet: .. Slides were then scanned using Axioscan Z1 Zeiss slide scanner (Carl Zeiss Microscopy GmbH) and images were analyzed with the Zen 2.6 software, or viewed on a Nikon A1 R confocal microscope (Nikon Instruments Inc), and images were taken using NIS elements AR software, and further processed using Fiji (Image J). .. Mucins from mice distal colonic tissues were purified by density gradient ultracentrifugation at 417,600 g for 72 h (Beckman Coulter 70.1 Ti rotor) after solubilization of tissues in a 4 M guanidine chloride buffer containing protease inhibitors (5 mm ethylenediaminetetraacetic acid, 0.1 mg/mL trypsin inhibitor and 1 mm phenylmethanesulfonyl fluoride) for 24 h. Mucin fractions were further submitted to β-elimination under reductive conditions (0.1 M NaOH, 1 M BH 4 Na for 24 h at 45°C).

    Article Title: Induction of intercrypt goblet cells upon bacterial infection: a promising therapeutic target to restore the mucosal barrier.
    Article Snippet: .. Slides were then scanned using Axioscan Z1 Zeiss slide scanner (Carl Zeiss Microscopy GmbH) and images were analyzed with the Zen 2.6 software, or viewed on a Nikon A1 R confocal microscope (Nikon Instruments Inc), and images were taken using NIS elements AR software, and further processed using Fiji (Image J).21 Mucin glycosylation analysis from mice colons Mucins from mice distal colonic tissues were purified by density gradient ultracentrifugation at 417,600 g for 72 h (Beckman Coulter 70.1 Ti rotor) after solubilization of tissues in a 4 M guanidine chloride buffer containing protease inhibitors (5 mm ethylenediaminetetraacetic acid, 0.1 mg/mL trypsin inhibitor and 1 mm phenylmethanesulfonyl fluoride) for 24 h. Mucin fractions were further submitted to β-elimination under reductive conditions (0.1 M NaOH, 1 M BH4Na for 24 h at 45°C). .. Released O-glycans were then permethylated in 200 μL dimethyl sulfoxide, 300 μL iodomethane and 1 g NaOH, for 2 h, and purified on a C18 Sep-Pak column (Oasis HLB, Waters).

    Microscopy:

    Article Title: Induction of intercrypt goblet cells upon bacterial infection
    Article Snippet: .. Slides were then scanned using Axioscan Z1 Zeiss slide scanner (Carl Zeiss Microscopy GmbH) and images were analyzed with the Zen 2.6 software, or viewed on a Nikon A1 R confocal microscope (Nikon Instruments Inc), and images were taken using NIS elements AR software, and further processed using FiJI (Image J) . .. Mice distal colonic tissues were solubilized in an extraction buffer containing 4 M guanidine chloride, 5 mM ethylenediaminetetraacetic acid (EDTA), 10 mM benzamidine, 5 mM oN-ethylmaleimide, 0.1 mg/mL trypsin inhibitor and 1 mM phenylmethanesulfonyl fluoride) for 24h, mucins were purified by density gradient ultracentrifugation at 417,600 g at 15 °C for 72 h (Beckman Coulter LE80 K ultracentrifuge; 70.1 Ti rotor), and identified by Periodic Acid Schiff (PAS) staining after transfer of the different fractions onto a nitrocellulose membrane by slot-blotting.

    Article Title: C. elegans transgenerational avoidance of P. fluorescens is mediated by the Pfs1 sRNA and vab-1 .
    Article Snippet: .. All images were taken on a Nikon Eclipse Ti microscope or Nikon A1 R confocal microscope. ..

    Article Title: Induction of intercrypt goblet cells upon bacterial infection: a promising therapeutic target to restore the mucosal barrier
    Article Snippet: .. Slides were then scanned using Axioscan Z1 Zeiss slide scanner (Carl Zeiss Microscopy GmbH) and images were analyzed with the Zen 2.6 software, or viewed on a Nikon A1 R confocal microscope (Nikon Instruments Inc), and images were taken using NIS elements AR software, and further processed using Fiji (Image J). .. Mucins from mice distal colonic tissues were purified by density gradient ultracentrifugation at 417,600 g for 72 h (Beckman Coulter 70.1 Ti rotor) after solubilization of tissues in a 4 M guanidine chloride buffer containing protease inhibitors (5 mm ethylenediaminetetraacetic acid, 0.1 mg/mL trypsin inhibitor and 1 mm phenylmethanesulfonyl fluoride) for 24 h. Mucin fractions were further submitted to β-elimination under reductive conditions (0.1 M NaOH, 1 M BH 4 Na for 24 h at 45°C).

    Article Title: C. elegans transgenerational avoidance of P. fluorescens is mediated by the Pfs1 sRNA and vab-1
    Article Snippet: .. All images were taken on a Nikon Eclipse Ti microscope or Nikon A1 R confocal microscope. ..

    Article Title: Induction of intercrypt goblet cells upon bacterial infection: a promising therapeutic target to restore the mucosal barrier.
    Article Snippet: .. Slides were then scanned using Axioscan Z1 Zeiss slide scanner (Carl Zeiss Microscopy GmbH) and images were analyzed with the Zen 2.6 software, or viewed on a Nikon A1 R confocal microscope (Nikon Instruments Inc), and images were taken using NIS elements AR software, and further processed using Fiji (Image J).21 Mucin glycosylation analysis from mice colons Mucins from mice distal colonic tissues were purified by density gradient ultracentrifugation at 417,600 g for 72 h (Beckman Coulter 70.1 Ti rotor) after solubilization of tissues in a 4 M guanidine chloride buffer containing protease inhibitors (5 mm ethylenediaminetetraacetic acid, 0.1 mg/mL trypsin inhibitor and 1 mm phenylmethanesulfonyl fluoride) for 24 h. Mucin fractions were further submitted to β-elimination under reductive conditions (0.1 M NaOH, 1 M BH4Na for 24 h at 45°C). .. Released O-glycans were then permethylated in 200 μL dimethyl sulfoxide, 300 μL iodomethane and 1 g NaOH, for 2 h, and purified on a C18 Sep-Pak column (Oasis HLB, Waters).

    Article Title: Developmental and quantitative expression profile of the six pollen allergens of mugwort (Artemisia vulgaris L.).
    Article Snippet: .. The pollen developmental stages were confirmed under Nikon A1 R confocal microscope, with a x40 magnification objective. ..

    Article Title: Developmental and quantitative expression profile of the six pollen allergens of mugwort ( Artemisia vulgaris L.)
    Article Snippet: .. The pollen developmental stages were confirmed under Nikon A1 R confocal microscope, with a x40 magnification objective. ..

    Article Title: Pseudomonas fluorescens 15 small RNA Pfs1 mediates transgenerational epigenetic inheritance of pathogen avoidance in C. elegans through the Ephrin receptor VAB-1
    Article Snippet: .. All images were taken on a Nikon Eclipse Ti microscope or Nikon A1 R confocal microscope. ..

    Glycoproteomics:

    Article Title: Induction of intercrypt goblet cells upon bacterial infection: a promising therapeutic target to restore the mucosal barrier.
    Article Snippet: .. Slides were then scanned using Axioscan Z1 Zeiss slide scanner (Carl Zeiss Microscopy GmbH) and images were analyzed with the Zen 2.6 software, or viewed on a Nikon A1 R confocal microscope (Nikon Instruments Inc), and images were taken using NIS elements AR software, and further processed using Fiji (Image J).21 Mucin glycosylation analysis from mice colons Mucins from mice distal colonic tissues were purified by density gradient ultracentrifugation at 417,600 g for 72 h (Beckman Coulter 70.1 Ti rotor) after solubilization of tissues in a 4 M guanidine chloride buffer containing protease inhibitors (5 mm ethylenediaminetetraacetic acid, 0.1 mg/mL trypsin inhibitor and 1 mm phenylmethanesulfonyl fluoride) for 24 h. Mucin fractions were further submitted to β-elimination under reductive conditions (0.1 M NaOH, 1 M BH4Na for 24 h at 45°C). .. Released O-glycans were then permethylated in 200 μL dimethyl sulfoxide, 300 μL iodomethane and 1 g NaOH, for 2 h, and purified on a C18 Sep-Pak column (Oasis HLB, Waters).

    Purification:

    Article Title: Induction of intercrypt goblet cells upon bacterial infection: a promising therapeutic target to restore the mucosal barrier.
    Article Snippet: .. Slides were then scanned using Axioscan Z1 Zeiss slide scanner (Carl Zeiss Microscopy GmbH) and images were analyzed with the Zen 2.6 software, or viewed on a Nikon A1 R confocal microscope (Nikon Instruments Inc), and images were taken using NIS elements AR software, and further processed using Fiji (Image J).21 Mucin glycosylation analysis from mice colons Mucins from mice distal colonic tissues were purified by density gradient ultracentrifugation at 417,600 g for 72 h (Beckman Coulter 70.1 Ti rotor) after solubilization of tissues in a 4 M guanidine chloride buffer containing protease inhibitors (5 mm ethylenediaminetetraacetic acid, 0.1 mg/mL trypsin inhibitor and 1 mm phenylmethanesulfonyl fluoride) for 24 h. Mucin fractions were further submitted to β-elimination under reductive conditions (0.1 M NaOH, 1 M BH4Na for 24 h at 45°C). .. Released O-glycans were then permethylated in 200 μL dimethyl sulfoxide, 300 μL iodomethane and 1 g NaOH, for 2 h, and purified on a C18 Sep-Pak column (Oasis HLB, Waters).



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